ADVANCES IN 3-DIMENSIONAL ELECTRON MICROSCOPY
三维电子显微镜方法进展

A great progress has been made in reconstruction of the three dimensional structure of cell and macromolecule from the electron micrograms due to the concurrently developments in the preparation of specimen for cro-electron microscopy, electron microscopic instrument and image processing and analysis. This has opened the avenues for cell biologist to explore the relationship between related organelles in the three dimensional space in complex cellular processes. This also enables the molecular biologist to analyze the structure up to high resolution, not only in the specimen which may form two dimensional crystal, but also in those macromolecules or macromolecule assemblies which have important biological function but unable to be crystallized.