MODELING OF scFv OF HUMAN ANTIBODY AGAINST LIVER CANCER
肝癌细胞特异性结合单链抗体三维结构的同源模建

3D Graphic model of two single-chains Fv (scFv) , named A4 and A10, against liver cancer was constructed by computer aided molecular design software. The VH (heave variable region) and VL(light variable region) domains of scFv were modeled respectively and then the whole three-dimensional structure of scFv was constructed. Based on these, the whole three-dimensional structure was refined by the methods of molecular mechanics and dynamics. It was proved by Profile-3D program to the structure of scFv was reasonable reliable. Computer graphic modeling indicated that all places of the linker, variable region of the heave (VH) and light (VL) chains were suitable. The VH region, as well as the VL region, was involved in composing the "hydrophotic pocket". The linker was isolated from VH and VL regions. The complementary determinant regions (CDRs) of VH and VL regions surrounded the "hydrophobic pocket". This study provides a structural basis for analyzing the functions of scFv and developing bispecific antibody with high affinity and specificity.