Calcium imaging of in-vitro proliferation and differentiation of neural stem cells
神经干细胞体外增殖分化的钙成像研究

Neural stem cells(NSCs), for their multipotency, have become a promising star in cell replacement therapy. However, their clinical use is restricted because of the poor knowledge of the intracellular and extracellular dues contributing to the proliferation and differentiation of NSCs. Recent studies showed that Ca2 signals may regulate these processes. In this research, calcium imaging technique was used to observe the process that single NSC proliferated and/or differentiated in vitro. The results implied that the calcium signals in NSCs were appeared in both proliferation and differentiation process, and in proliferation, calcium transients are the accompanying signals reflecting cellular events, while in differentiation, the dependent signals associated with cell fate tightly. Eventually, it hypothesized a possible mechanism that how Ca2 signals regulate the process of the differentiation of NSCs and pointed out the perspective of future research.