ESR STUDIES ON THE EFFECTS OF GPCS TREATED LIPOSOMES ON SUPEROXIDE-GENERAIDN OF RAT NEUTROPHILS
用ESR自旋捕集法研究吸烟烟气处理的脂质体对大鼠粒细胞产生O_2~-的影响

Gas-phase cigarette smoke (GPCS) was capable of inducing lipid peroxid-ation in lecithin-formed liposomes. When intact rat peritoneal neutroghils (RPN) were treated by the liposomes which had been bubbled by GPCS for 20 seconds, it was found, by using ESR spin trapping technique, that these pe-roxidized lipids enhanced the generation of superoxide anions in RPNs, if the duration time was within 25 minutes (1.0 mg/ml lipids) or the concentration of lipids was less than 15.0 nig/nil (the duration time was 15 minutes). While, the newly-prepared liposomes without GPCS-treating showed an inhibiting effect on the activity of RPNs when the concentration of lipids was more than 0.2mg/ml (the duration time was 15 minutes).