THE ULTRAVIOLET FLUORESCENCE AND INTRAMOLECULAR ENERGY TRANSFER OF PURPLE MEMBRANE
紫膜的紫外荧光及分子内的能量转移

The UV fluorescence spectra of purple membrane fragments were measured. The fluorescence intensity of protein in the PM samples irradiated with orange light was lower than that of control. The intensities of UV fluorescence of PM and apomembrane were similar, but the quantum yield of UV fluorescence of bacterioopsin was twice as large as that of the natural purple membrane. This result illustrated that the protein fluorescence was possibly quenched by the chromophore of PM. The conversion of the dark-adapted PM into light-adapted PM was induced by irradiation of UV light at 280nm. The photobleaching of PM was also induced by the UV light in the presence of hydroxylamine. By measuring the action spectrum of photobleaching of PM we have showed that the action spectrum of photobleaching of PM coincided with the absorption spectrum of PM in the UV region.All the results showed that the energy absorbed by protein had transferred to retinal.