THE QUANTITATIVE STUDY ON MICROVASCULARPERMEABILITY UNDER POST-ISCHEMIC REPERFUSION
定量研究脑缺血及再灌注下微血管的通透性

The rat model of cerebral ischemia with reperfusion was employed to observe intravital microvessels and to measure the fluorescence intensities of blood, brain and other five internal organs of the body for studying the permeability of cerebral microvessels with fluorescent tracer FLNa. It was designed to insure that the image of cerebral microvessels was smoothly processed and was quantitatively analyzed in order to find the effect and inherent law of ischemia with reperfusion on the permeability of cerebral microvessels. Experiment results show that ischemia and ischemia with reperfusion do harm on endothelial cells of the capillary;In this case the permeability was obviously enhanced as compared with that in normal condition, and the long duration of reperfusion is helpful to trauma repairing. Under the same conditions, their different fluorescence intensities show that their resistance to ischemia and lacking of oxygen isn't identical, and the degree of harm varies considerably in internal organs, where the most serious is the endothelial cells of liver and kidney, the lesser those of brain and spleen. The condition of lung is an exception and the speciality of lung is related to its structure. Besides, the method of image process and quantitative analysis was established to find diffuse equation and diffuse speed of fluorescence agent after passing through the wall of microvessels.