STUDY ON THE MODEL OF ZINC ABSORPTION KINETICS WITH RAT SMALL INTESTINE
小肠锌吸收动力学模型的研究

A model of zinc absorption kinetics was studied with rat small intestine. The jejunum and ileum were perfused in situ with 0.85% saline containing 0.1 mM ZnSO4. The zinc concentrations of perfusate were analysed by atomic absorption spectrophotometer (AAS). A two-compartment model was used to describe the kinetics of zinc absorption and to determine the kinetic constants. Compare between results calculated by the model and that determined by AAS was made. To test the availability of the model, the influence of androgen on kinetics of zinc absorption of small intestine was studied with the castrated rats. Following results were noted: (l)the relative errors between zinc concentrations of intestinal mucosa calculated by the model and that determined by AAS are smaller than 9%, and (2) the model can qantitively describe the kinetics of zinc absorption and compare between kinetic constants can be made. The results show that the model can satisfactorily reflect the kinetics of zinc absorption of intestine and is an effective new method for study of the mechanism of zinc absorption.