Gene expression profile of rat mesenchymal stem cell spontaneous transformation determined by cDNA microarray analysis
芯片分析自发恶性转化的大鼠骨髓间充质干细胞的基因表达谱

To study the genetic basis of spontaneous transformation of mesenchymal stem cells(MSCs) and clinical application value MSCs were isolated by combining utilization of density gradient centrifugation and adherence screening.After cell homogeneity analysis by flow cytometry,spontaneous transformation MSCs were isolated after six-month in vitro expansion.Then cell total RNA was obtained with Trizol reagent and studied for gene expression profile.Differentially expressed genes between normal MSCs and spontaneou...