Synthesis of glucose laurate monoester catalyzed by Candida antarctica lipase B-displaying Pichia pastoris whole-cells
毕赤酵母表面展示南极假丝酵母脂肪酶B全细胞催化合成葡萄糖月桂酸酯

We developed a new enzymatic-catalyzing producing process of glucose laurate monoester.In the process we used Candida antarctica lipase B-displaying Pichia pastoris whole-cells as biocatalyst,glucose as the acyl acceptor and lauric acid as the acyl donor.The product glucose laurate monoester was purified by silica gel column chromatography and preparative liquid chromatography,and identified by liquid chromatography-mass spectrometry.Then we optimized the process from various aspects,such as solvent composi...