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Cloning and characterization of porcine NOBOX gene
NOBOX基因的cDNA克隆及其特性分析

Keywords: pig,NOBOX gene,cDNA cloning,bioinformatics analysis,gene expression
,NOBOX基因,cDNA克隆,生物信息学分析,基因表达

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Abstract:

Newborn ovary homeobox gene(NOBOX) is an oocyte-specific homeobox gene that plays a critical role in early folliculogenesis and represents a candidate gene for nonsyndromic ovarian failure.We used in silico approach in combination with rapid amplification of cDNA ends(RACE) to clone the full-length cDNA of NOBOX(GenBank Accession No.FJ587509) from porcine oocytes.It contains 1768 bp nucleotides,with an open reading frame(ORF) of 1419 bp.The putative porcine NOBOX gene encodes 472 amino acids with the molecu...

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