Expression and analysis of the recombinant human interleukin-21 (rhIL-21) in Pichia pastoris
重组人白细胞介素21在毕赤酵母中的分泌表达及活性分析

Interleukin-21 is a type I cytokine mainly produced by activated CD4+ T cells that acts as a regulator of immune system.In this work,hIL-21cDNA was amplified from human peripheral blood lymphocytes by RT-PCR,and then inserted into pPIC9K.The recombinant vector pPIC9K-hIL21cDNA was linearized by Sac I,and transformed into Pichia pastoris strain GS115 by electroporation.Transformants were selected by G418 and confirmed by PCR.The recombinant protein was expressed and secreted into the supernatant after induci...