Recombinant expression of the fusion antigen based on Treponema pallidum TpN17 and TpN47 epitope peptides and establishment and application of the associated ELISA
梅毒螺旋体TpN17与TpN47表位肽融合抗原的重组表达及其酶联免疫吸附试验建立和应用

Using recombinant TpNs proteins of Treponema pallidum as antigens,ELISAs are proved to be of higher sensitivity and Journals.im.ac.cn specificity.However,they can be further increased by using multiple TpNs antigens.According to the epitope analysis,we firstly used linking primers PCRs to obtain an artificial fusion gene segment tpE17-47 containing epitopes of both TpN17 and TpN47.Subsequently,we conducted the prokaryotic expression systems of entire tpN17 and tpN47 genes and tpE17-47 fusion gene.SDS-PAGE a...