THE APPLICATION OF SYNCHROTRON RAIATION IN DIFFRACT ON DATA COLLECTION OF D-GLYCERALDEHYDE-3-PHOSPHAIE DEHVDROGENASE AND IT''S DERIVATIVES
同步辐射在D-甘油醛-3-磷酸脱氢酶及其系列衍生物高分辨率衍射数据收集中的应用

Helo - D - glyceraldehyde - 3 - phosphate dehyregenase HOLO - GAPDUwas purified frorr P. versicolor lobster muscle. It's active site Cys-149 carboxymethy and den-vative CM-GAPDH) and the fluorescent NAD derivative IRR-GAPDH were prepared. Using I (NH4) 2SO4 as precipitating agent, the three forms of the en me were crystallized by vapour diffusion method. The diffraction data of these three forms of the enzyme were collected with synchrotron radiation-Fuji imaging plate -weisse berg cam-era system at BL-6A2 of Photon Factory at 1.8A resolution. Rmerge factor of HOLO-GAPDH, CM-GAPDH and IRR-GAPDH are 4.93%, 6.22% and 5.77% respectively. The diffraction intensity difference between the native enzyme and it's derivatives and its structural implication arc discussed.