Cloning of cDNAs encoding skin antimicrobial peptide precursors from Chinese brown frogs, Rana chensinensis and determination of antimicrobial, anticancer and haemolysis activity
中国林蛙皮肤抗菌肽基因的cDNA克隆及抗菌、抗癌和溶血活性的测定

Amphibian skin antimicrobial peptides exhibit a broad spectrum of antimicrobial activity against Gram-positive and Gram-negative bacterium and cytotoxic activity responsible for inhibiting the growth of cancer cells.In this present study,six cDNAs encoding antimicrobial peptide precursors were cloned from the skin of Chinese brown frog,Rana chensinensis by RT-PCR and 3'-RACE procedure and identified as preprotemporin-1CEa,preprotemporin-1CEb,preprotemporin-1CEc,preprobrevinin-1CEa,preprobrevinin-1CEb,and pr...