Prokaryotic expression of recombinant Streptococcus suis IgG binding protein and its binding activity with IgG
猪链球菌IgG结合蛋白的原核表达及其与不同动物IgG的结合性

Streptococcus suis (S. suis) IgG-binding protein (SPG) was present in all S. suis strains examined. It showed binding activities with IgG from various host species. Little was known about the biological role of this protein,but it was commonly believed that it acted as virulence factor. In this study,the genes encoding SPG were amplified respectively from the total DNA of the S. suis serotype 1/2,1,2 and 9 with PCR and expressed in Escherichia coli BL21 by plasmid pET28a as vector. The recombinant proteins ...