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生物物理学报 2002
EFFECT OF CHOLINE CHLORIDE ON MEMBRANE FLUIDITY OF MITOCONDRIA ISOLATED FROM ETIOLATED SEEDLINGS OF WHEAT
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Abstract:
Effects of choline chloride (CC) on fluorescence intensity, micro-viscosity (η) and ESR spectra of mitochondria from wheat etiolated seedlings were studied with the fluorescence labels, ANS and DPH and a spin label, 16NS. Results indicated that the concentration of CC from 0.21 to 1.79 mmol·L-1 decreased fluorescence intensity, micro-viscosity (η), sequence parameter (S) and rotating correlative time (τc) of mitochondria from etiolated wheat seedlings. CC could therefore increase the fluidity of mitochondrial membrane. All these results suggested that CC could affect the biophysical characteristics and functions of mitochondria, which shed some light on the mechanism of increased resistances against stresses in plants by CC.