Intrinsic prokaryotic promoter activity of SUMO gene and its applications in the protein expression system of Escherichia coli
SUMO基因的内在原核启动子活性及其在大肠杆菌蛋白表达系统中的应用

Nowadays,SUMO fusion system is important for recombinant protein production in Escherichia coli,yet a few aspects remain to be improved,including the efficacy for vector construction and protein solubility.In this study,we found the SUMO gene Smt3(Sm) of Saccharomyces cerevisiae conferred an unexpected activity of constitutive prokaryotic promoter during its PCR cloning,and the gene coding regions of SUMOs in most species had a ?70-dependent prokaryotic promoter embedded,through the prediction via the BPROM...