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OALib Journal期刊
ISSN: 2333-9721
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A multipathogen selective enrichment broth (SVV) for simultaneous growth of Salmonella, Vibrio parahaemolyticus, and Vibrio cholerae
选择性富集沙门氏菌、副溶血弧菌和霍乱弧菌的共增菌培养基SVV

Keywords: multipathogen selective enrichment broth,SVV,Salmonella,Vibrio parahaemolyticus,Vibrio cholerae
选择性共增菌肉汤
,SVV,沙门氏菌,副溶血弧菌,霍乱弧菌

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Abstract:

We formulated a selective enrichment broth (SVV) for simultaneous growth of Salmonella, Vibrio parahaemolyticus, and Vibrio cholerae by single factor experiment and response surface method. We evaluated the enrichment effect of SVV by conventional culture method and real-time PCR assay. We obtained the SVV broth by supplementting the Buffered Peptone Water (BPW) with bile salt no. 3, potassium tellurite, and sodium citrate as inhibitors, and glucose, mannitol, snhydrous sodium sulfite and sodium pyruvate as...

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