Expression, purification and characterization of HbsAg binding protein in Pichia pastoris
乙肝表面抗原结合蛋白SBP在毕赤酵母中的表达纯化及功能鉴定

Human hepatitis B virus surface antigen (HBsAg) binding protein(SBP) shows a specific binding ability to HBV surface antigen HBsAg. Previous work proved an ability of SBP to enhance the immune response of HBsAg vaccine. To investigate the function and mechanism of this protein, we constructed SBP-expression strains with Pichia pastoris expression system. We screened these strains and have got an expression strain with high protein expression quantity. Fermentation product was collected and purified to gain ...