Application of metabolic flux ratio analysis in metabolic engineering-a review
代谢流量比率分析及其在代谢工程中的应用

Intracellular metabolic fluxes are important to understand metabolic characteristics of cells and to direct metabolic engineering strategies.Because intracellular fluxes cannot be directly assessed,isotope experiments are usually conducted to trace metabolic fluxes.The flux-ratio analysis can reflect high biochemical veracity,be employed to identify the topology of the networks,and offer greatly reduced computational expense for flux determination.In order to apply this metabolic analysis method to better e...