Effect of the chemically assisted enucleation on the enucleation of sheep oocytes and the development of their reconstructed embryos
化学辅助去核法对绵羊卵母细胞去核率和重构胚发育率的影响

In order to enhance the efficiency of sheep somatic cell nuclear transfer,we used a chemically assisted enucleation with colchicine to study the effects of the concentration of colchicine,the incubation time of oocytes in colchicine and the maturation time of oocytes on the enucleation rates and the development of reconstructed embryos. The results showed that 1) there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 ...