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生物工程学报 2009
Identification of the regulation elements in heat-inducible Lehsp23.8 promoter
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Abstract:
The promoter of mitochondria-localized small heat shock protein gene in Lycopersicon esculentum(Lehsp23.8) is characterized as strongly heat-inducible.In this study,to determine how the expression of Lehsp23.8 is regulated,we conducted five expression vectors carrying the gus gene driven by the 5' deletion products of the Lehsp23.8 promoter.The corresponding transgenic tobacco plants were generated via Agrobacterium tumefaciens-mediated transformation.Transgenic plants were identified by PCR and Southern bl...
