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生物工程学报 2009
Morphological observation of Bovine Kidney (MDBK) cells effected by foot-and-mouth disease virus Lpro
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Abstract:
In order to explore the morphological changes of Bovine Kidney(MDBK) cells induced by foot-and-mouth disease virus(FMDV) L protease,we induced the expression of FMDV L protease in bovine kidney cells(MDBK) artificially.All work is carried out on the basis of a stable MDBK cell line inducibly expresses the Lab gene under the control of tetracycline.We use cell morphology,Hoechst 33258 staining,AO-EB staining,and DNA Ladder abstraction to research the morphological changes of MDBK cells.24 hours after FMDV L ...