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ISSN: 2333-9721
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Morphological observation of Bovine Kidney (MDBK) cells effected by foot-and-mouth disease virus Lpro
口蹄疫病毒前导蛋白(Lpro)致牛肾细胞(MDBK)作用的形态学观察

Keywords: foot-and-mouth disease virus,L protease,morphological changes of Bovine Kidney,apoptosis
口蹄疫病毒
,Lpro,MDBK,细胞凋亡

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Abstract:

In order to explore the morphological changes of Bovine Kidney(MDBK) cells induced by foot-and-mouth disease virus(FMDV) L protease,we induced the expression of FMDV L protease in bovine kidney cells(MDBK) artificially.All work is carried out on the basis of a stable MDBK cell line inducibly expresses the Lab gene under the control of tetracycline.We use cell morphology,Hoechst 33258 staining,AO-EB staining,and DNA Ladder abstraction to research the morphological changes of MDBK cells.24 hours after FMDV L ...

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