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生物工程学报 2009
Recent advances in enzyme assays using fluoremetry
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Abstract:
Enzymes play such a pivotal role in cellular metabolism that enzyme assays are important for bio-engineering,disease diagnoses and drug discovery.Among the reported methods,fluoremetry has attracted more and more attention due to its high sensitivity and possibility of continuous dynamic monitoring.The recent progresses and applications in enzyme assays using fluorescent probes were reviewed.Different methods were classified into direct fluorescence detection and indirect fluorescence detection according to...