Immunogenicity of Staphylococcus aureus recombinant clumping factor A
金黄色葡萄球菌凝集因子A的免疫原性

In order to characterize the immunogenicity and immunoprotection of the Staphylococcus aureus(S.aureus) surface protein Clumping factor A(ClfA),we amplified clfa genes from S.aureus Newman strain,Wood46 strain and HLJ23-1.The clfa gene from Newman strain was subsequently inserted into pQE-30 vector and the recombinant plasmid was transformed into Escherichia coli strain M15(pREP4).The recombinant ClfA protein was expressed and purified.Then,we immunized mice with the purified recombinant protein.The antibod...