|
生物工程学报 2009
Differentiation of bovine male germ-line stem cells in vitro
|
Abstract:
Male germ-line stem cells(mGSCs) have the capability of self-renewal and latent capability of differentiation.mGSCs is the unique diploid immortal cell which can transfer genetic information to filial generation.The combination of transgenic technology and mGSCs heterotransplanting will supply new opportunities and paths to cloning animal,transgenic animal and gene therapy of some human hereditary disease.We studied the isolation and cultivation of mGSCs that were isolated and purified from 5?6 month old bo...