Construction and immunogenicity of recombinant adenovirus co-expressing the GP5 and M protein of porcine reproduction and respriratory syndrome virus in mice
表达猪繁殖与呼吸综合征病毒GP5和M融合蛋白的重组腺病毒的构建及其对小鼠的免疫原性

FMDV 2A peptide was introduced as a linker between GP5 and M protein of porcine reproduction and respiratory syndrome virus (PRRSV) to allow automatic self-cleavage the polyproteins. This strategy simultaneously displayed the neutralizing action of GP5 protein and cell-mediated immunity of M protein. We put them into the expression cassette of adenovirus vector. The results of RT-PCR,IFA and Western blotting showed that GP5 and M protein were not only expressed correctly,but also self-cleavaged and assemble...