Prolonged-releasing Performance of Drug Loaded Polyelectrolyte Nanocapsules Effected by Acid Phosphatease: in vitro Analysis
生物响应酸性磷酸酶浓度的载药聚电解质的体外控释分析

The aims of the study were to prepare polyelectrolyte nanocapsules effected by acid phosphatease (ACP) and to study prolonged-releasing performance of the nanocapsules in vitro. Using the layer by layer (LbL) self-assembly technique, polyelectrolyte-b-glycerophosphoric acid nanocapsules were prepared. The morphologies of the nanocapsules were characterized by transmission electron microscopy (TEM) and biocompatibility was well examined by cell-culture method. The drug adriamycin would be loaded in nanocapsules for concentration gradient, the encapsulation efficiency could be calculated. Nanocapsules were reacted with acid phosphatease standard and HepG2 cells that express the ACP, respectively. The prolonged-releasing of adriamycin was verified and tumor cells apoptosis were measured. TEM images showed that the nanocapsule sizes were between 200~300 nm. The material biocompatibility was good until the concentration of nanacapsule was up to 250 mg/mL. The drug encapsulation efficiency reached 68.12%. The release rate of polyelectrolyte (PAH/PSS-b-glycerophosphoric acid)s nanocapsules was higher than in the control nanocapsules at 48h(38% Vs 15%) after its reaction to the ACP standard(P<0.05). Compared with the control, nanocapsules could significantly inhibit the growth of HepG2 cells that expressed the ACP, and the efficiency of cell apoptosis was 7.59% higher at 24h (13.73 Vs 6.14, P<0.05). Polyelectrolytes (PAH/PSS-b-glycerophosphoric acid) nanocapsules in vitro have response to acid phosphatease by which prolonged-releasing can be affected. This property can be used for treatment of some malignant and benign diseases with elevated acid phosphatease level.