Effects of extracellular matrix produced in vitro on growth and differentiation of MC3T3-E1 cells
体外培养所形成的细胞外基质对MC3T3-E1细胞生长和分化的影响

Extracellular matrix(ECM) keeps cell's shape,protects and nourishes cells;it plays a great role in cell proliferation and differentiation.Therefore,ECM is very important in cell and tissue engineering.In this study,after primary mouse osteoblasts and fibroblasts maintained at confluence in vitro were removed,their ECM coated on cell culture plate was prepared,and bone morphogenetic proteins 2(BMP-2) was detected in the osteoblasts ECM.MC3T3-E1 preosteoblasts cells were seeded on cell culture plates covered ...