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生物工程学报 2009
Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro
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Abstract:
We studied the effect of photodynamic therapy with phycobiliproteins on human liver cancer cells in vitro.With 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay(MTT assay),we used two phycobiliproteins,R-phycoerythrin(R-PE) and C-phycocyanin(C-PC) prepared from Porphyra yezoensis,to determine the killing rates and apoptosis rates of human liver cancer cells(SMMC-7721) mediated by laser.When the concentration of R-PE was 120 mg/L,the survival rate of human liver cancer cells was 27% after tr...