Molecular design and immunogenicity of a multiple-epitope foot-and-mouth disease virus antigen, adjuvants, and DNA vaccination
口蹄疫病毒三价复合多表位佐剂DNA疫苗构建及其免疫原性

We designed and constructed a fuse expression gene OAAT and staphylococcal enterotoxin A (SEA) on the basis of the OAAT designed and constructed which consists of the structural protein VP1 genes from serotypes A and O FMDV,5 major VP1 immunodominant epitopes from two genotypes of Asia1 serotype,and 3 Th2 epitopes originating from the non-structural protein,3ABC gene and structural protein VP4 gene. The recombinant plasmids pEA was constructed using SEA as a genetic adjuvant. Expressions of target gene from...