Expression of heterogenous pyruvate carboxylase in Escherichia coli with lactose as inducer and its effect on succinate production
乳糖诱导丙酮酸羧化酶基因在大肠杆菌中的表达及对丁二酸产量的影响

Escherichia coli strain DC1515,deficient in glucose phosphotransferase(ptsG),lactate dehydrogenase(ldhA) and pyruvate:formate lyase(pflA),is a promising candidate for the fermentative production of succinate.To further improve the succinate producing capability of DC1515,we constructed plasmid pTrchisA-pyc with heterogenous pyruvate carboxylase(pyc) from Bacillus subtilis 168 under the Trc promoter and introduced it into DC1515.We used lactose as a substitute of IPTG to induce pyc.We optimized the culture c...