Effect of different carbon sources on pyruvic acid production by using lpdA gene knockout Escherichia coli
不同碳源对大肠杆菌lpdA突变菌累积丙酮酸的影响

We studied the ability of lpdA gene knockout Escherichia coli to ferment different sugars in mineral salts medium for the production of pyruvate.The sugars studied were glucose,fructose,xylose and mannose at a concentration of 10 g/L.At the same time,effect of inoculum size on lpdA fermentation with glucose was studied.The strain was able to use all sugars for biomass generation and pyruvate production.The lpdA knockout mutant converted glucose,fructose,xylose and mannose to pyruvate with yields of 0.884 g/...