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OALib Journal期刊
ISSN: 2333-9721
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Fusion Tags Technology and Their Applications
融合标签技术及其应用

Keywords: Fusion tags,Recombinant protein,oriented immobilization,visualization,solubility
融合标签
,重组蛋白质,定向固定,可视化,可溶性

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Abstract:

Fusion tags are originally developed to facilitate the purification of recombinant protein from crude extracts. In recent years, the discovery of different tags and the development of fusion strategy make the function of fusion tags diversified. However, there was no a cure-all fusion tag for different applications. We here give an overview of fusion tag technology and the different applications of fusion tags, including the purification, detection and oriented immobilization of recombinant protein, the visualization of bioevent in vivo, the enhancement of the yield of protein, the improvement of the solubility and stability of the expressed protein.

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