Expression of endothelin in organ-cultured artery under low shear stress
低切应力作用下体外培养动脉内皮细胞内皮素的表达

The biological effect of shear strees on intact artery and the change of Endothelin (ET) during the stress-induced vascular remodeling was studied. The common carotid arteries of pigs were cultured with a artery organ-culture system under different shear stress, 0.5 and 2 Pa respectively, 1.33×10-4 Pa of pressure and stable flow in vitro. Cultured for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h, the content of ET in the perfusate was examined with radio-immunity method, and the data were described by Logistic equation. The secretion velocity of ET didn't change obviously in S20 group, while in S5 group it rised evidently at the first 12 h and then descend to a stable level which was higher than S20 group all the time. The secretion and expression of ET rised under low shear stress. It may play an important role in vascular remodeling induced by low shear stress.