Comparative Study on in vitro Stationary Culture System or Superfusion Culture System of Cow Mammary Tissue
组织灌流与贴壁法体外培养奶牛乳腺组织的比较研究

Dairy cow mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and uhrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation. The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 12 -60h in DMEM plus 10% calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within 60 - 108h. Both culture systems had some advantages and disadvantages.