Heterologous Expression and Characterization of L-Amino Acid Deaminase from Proteus mirabilis in Escherichia coli
奇异变形杆菌的L-氨基酸脱氢酶在大肠杆菌中的异源表达和鉴定

We expressed an L-amino acid deaminase (Pma) from Proteus mirabilis in Escherichia coli and characterized the kinetics of phenylpyruvic acid production. P. mirabilis Pma was well expressed in E. coli in an active state and was found to be associated with membranes. The association of Pma with cellular membranes is likely to be necessary for its enzymatic activity.