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OALib Journal期刊
ISSN: 2333-9721
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Recombinant D-amino Acid Oxidase with Improved Properties
重组具有改良特性的D-氨基酸氧化酶

Keywords: D-amino acid oxidase,Trigonopsis variabilis,directed mutagenesis,X-ray structure
D-氨基酸氧化酶
,三角酵母,定向诱变,X射线结构

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Abstract:

D-amino acid oxidase from Trigonopsis variabilis (TvDAAO) was overproduced in E. coli cells and properties of the recombinant enzyme were studied. Single point mutants of the enzyme with 2.4-fold higher thermal stability or changed spectra of substrate specificity compared to wild-type enzyme were prepared. It was shown that mutant TvDAAO has higher catalytic efficiency in cephalosporin C oxidation in comparison with wild-type enzyme. One mutant of recombinant TvDAAO was crystallized and its structure was solved with resolution 2.8 ?.

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