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生物物理学报 1991
THE INFLUENCE OF PBS SUSPENDING MEDIUM ON EKTACYTOMETER OSMOSCAN CURVE
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Abstract:
Ektacytometer osmoscan ourve desoribing erythrocyte deformability index (DI) in'relation to Inoresing osmolality of the suspending buffer were studied and osmoscan curve for PBS suspending medium has been obtained for the first time. The authers found that different influences of high viscosity suspending medium (PBS) on Ektacytometer osmoscan curve exist. However, erythrocyte de-formabillty in both high osmotio suspending media increases with the time.The results read as follows:1 .Ektaoytometer osmosoan curve for PBS suspending mediun is different from that for dextran, no minimum DI exists in low osmotic range of Ektacytometer osmoscan curve, namely, the DI doesn't increase with decrease of osmotic pressure.2.DI in high osmotio pressure range of Ektaoytometer osmoscan curve for PBS suspending medium decreases slowly i.e. Ektacytometer curve in high osmotio range becomes broader than that for dextran suspending medium.3.Ektacytometric measurements interchanging for PBS suspending medium and dextran suspending medium testified that crythrooyte deformabiliy in PBS suspending medium is better than that in dextran suspending medium in the high osmotic pressure.