THE INFLUENCES OF EMP IRRADIATION OF RAT HYPOTHALAMUS IN VITRO ON INTRACELLULAR LDH AND LDH、 AST、 CHE、 K+ AND Na+ IN CULTURE MEDIUM
电磁脉冲辐照对培养的大鼠下丘脑神经细胞内外 LDH、AST、CHE、K+ 和Na+ 的影响

After rat hypothalamus was irradiated by electromagnetic pulse (EMP) in vitro the intracellar LDH and LDH?AST?CHE?K + and Na + in culture medium were measured to analyze the EMP radiation in-jury mechan ism of hypothalamus neurons. Method as follows: Hypothalamus neurons of wistan r at in neonatal moment were cultured in six 6-hole boards. One board was contro l sample and other five boards were irradiat-ed by high field strength 5 EMP (electromagnetic pulse,EMP) within 2 minutes. The electromagnetic pulse simulator provides 5 pulses/min with a high electric field intensity 60 KV/m, 20 nsec rise time and 30 s pulse width. The intracellular LDH of neuron and LDH, AST, CHE, K + and Na + in culture medium were measured at 0 h, 1 h, 6 h, 12 h and 24 h after the n eurons being irradiated, using reagent boxes(Beijing Zhongsheng high- tech Bio engineering Company). All data was analyzed by statistical software Sps s8.0. The results of measure-ment show intracellular LDH was decreased from 1 hour after irradiation and through to 12 hour, while LDH and AST in cul ture medium were increased at 0 hour after irradiation and at 1 h and 6 h were i ncreased signif-icantly CHE and K + in medium also increased significantly at 1 h after EMP irradiati on. Na + in medium in-creased at 6 h. Both K + and Na + at 12 h after EMP irradiation still high in medium. All these substanceas a t 24 hours after irradiated by EMP were restored to the originel value in no di fference with the control. The results suggest that EMP could injure the membra nes of hypothalamus neurons.