Hairy-root Culture of Polygonium multiflorum Thunb. and the Production of Its Active Constituents-anthraquinones
何首乌毛状根培养及其活性成分的产生

Transformed hairy roots of \%Polygonium multiflorum\% Thunb.were obtained by the transformation of \%Agrobacterium rhizogenes\% LBA9402.It was clearly demonstrated that T-DNA of \%A.Rhizogenes\% Ri plasmid was integrated into the cells of hairy roots by the experiments of PCR and Southern hybridization.Through the screen of basic medium and the research of growth curve of hairy roots,the optimum inoculum time was selected in 30 days or so in the optimum condition-MS medium.On the condition,the content of rhein was 2.85 folds higher in hairy roots than that of natural plants by means of HPLC.