STUDIES ON CAPACITY OF PICKING UP ELECTRONS ON THE SURFACEOF IRON CORE FORM THE FERRITIN OF FISH LIVER
鱼肝铁蛋白铁核表层接受电子能力的研究

The fast and slow rates of the reduction electrons on the surface of iron core in liver ferritin of Dasyatis akajei (DALF) were studied by direct electrochemical technology which was also used to study the kinetic order and law of iron release. In aerobic environment, the experimental results showed that DALF, as a form of biphasic behavior, not only picked the electrons up from the platinum electrode, but also used them for iron releasing. Moreover, two-reduction potential of -125 mV and -375mV vs. NHE for iron release were determined. Using reduction potentials of -200 mV and- 500mV vs. NHE, two rates of iron release on the surface of iron core in DALF measured were 11.1 Fe3 /DALF/min and 33.3Fe3 /DALF/ min respectively, suggesting that the fast and slow rates of DALF picking the electrons up from the platinum electrode are tightly related to the reduction potentials. It was seen that the heme not only bound to the protein shell of DALF, but also accelerated the rate of iron release. However, the content of iron release was not increased by the heme in DALFh. The structure composition of phosphate and iron within the DALF core were heterogeneous. The surface structure of iron core in DALF is capable of picking up the electrons come from the electron tunnel of protein shell, which are used for iron releasing.