Callus Induction and Regeneration from Mature Seeds of Indica Rice Minghui 63 and Anti-funga] Assay of Transgenic Rice Plants
籼稻明恢63成熟种子愈伤组织的诱导及转基因水稻的抗性检测

A large number of callus from mature seeds of indica rice minghui 63 were obtained through pre-induction on medium with 2mg/L 2,4-D but without inorganic and organic components for 9 days. Trichosanthin gene was transferred into indica rice minghui 63 by using agrobacterium with the help of bombardment and the transgenic plants were obtained by inducing regeneration. Southern and Western blot analysis showed that the trichosanthin gene had been transferred into genome of minghui 63 and expressed in rice plants. The anti-fungal assay suggested that transgenic rice plants enhanced resistance to infection of Pyricularia oryzae.