TO FIND NUCLEOTIDE BINDING SITES OF DBPS BY COMPARING THE PROMOTER SEQUENCES OF INSULIN GENE
利用序列比较寻找胰岛素基因表达启动区与DNA结合蛋白的结合位点

There were several DNA binding proteins (DBPs) related to insulin gene expression, such as NDF1, IPF1 and HNF4. The structures of three DBPs was homologous by comparing the amino acid sequences, motifs and domains of human, mouse and rat in SWISSPROT protein database. According to the relationships between structure and function of protein, it was supposed that the nucleotide binding sites of DBPs were similar in the promoters of insulin gene. The DNA sequences of human, mouse and rat were obtained in GenBank nuclear database. The ClustalW program of multiple alignment was used to compare nucleotide sequences of the three mammals. It showed that there were some similar se-quences in the promoters of insulin gene. In the meanwhile, the nucleotide binding sites of NDF1, IPF1 and HNF4 were searched in the TRANSFAC gene regulation database. The conservative nucleotide se-quences were found between the promoters alignment of insulin gene and the nucleotide binding sites of DBPs in the TRANSFAC database. Some other conservative nucleotide sequences in the alignment were perhaps the binding sites of unknown proteins. This device of sequences alignment offered a simple and applicable method to find and confirm the nucleotide binding sites of DBPs in the molecular biology ex-periments.