A T7-promoter Based Escherichia coli Expression System Induced with Bacteriophage M13HEP
利用重组噬菌体诱导表达的大肠杆菌表达系统

The bacteriophage M13HEP which can express T7 RNA polymerase under the control of lac promoter was constructed by cloning the T7 RNA polymerase gene into phage M13mp18 RF DNA. Heterologus genes under the control of T7 promoter can be induced by introducing T7 RNA polymerase to the cells through M13HEP phage infection. Many heterologus genes were successfully expressed by using this phage M13HEP induction system, especially some gene products to be expressed are toxic to host strain BL21 (DE3). A new E. coll strain HMS174F' was also constructed by transfer F'pilli from E. coli XL1-blue to E. colt HMS174. It made T7 expression plasmid construction , expression and single strand DNA rescue can be performed in the same E. coli strain.