Effects of Low Concentration Glutaraldehyde on Biomechanical Properties of Erythrocyte Membrane
低浓度戊二醛对红细胞膜力学特性的影响

The purpose of the study was to perform noninvasive, in situs, real time measurements on the mechanical properties of young and senescent red blood cell (RBC) membrane as a function of glutaraldehyde concentration in a low concentration range below 0.005%, and the incubation time, by the means of micro-dynamic image analyzing technique. The results showed that: 1) The Kc's of both 'young' and 'old' RBC membrane increased obviously with increasing glutaraldehyde concentration, and the Kc of 'old' RBC membrane was higher than that of 'young' RBC membrane incubated with the same time. 2) The difference of Kc's for 'young' and 'old' RBC membrane increased with incubation time. This suggests that, glutaraldehyde has different effects on 'young' and 'old' RBC membrane sketelon; and 'young' RBC membrane will be more efficient as carrier of therapeutic agent. On the other hand, the results provide reference for choosing proper glutaraldehyde concentration in immunochemical experiments.