Two Interspecific Somatic Hybrid Plants Regenerated Via Protoplast Electro-fusion
柑桔细胞电融合再生两个种间体细胞杂种

Protoplasts isolated from cell suspension cultures of 'Bonnaza' navel orange (Citrus sinensis L. Osbeck) were electrically fused with mesophyll protoplasts of rough lemon (Citrus jambhiri Lush) and Goutou orange (Citrus aurantium L.) respectively. Plants regenerated from both fusion combinations. Chromosome counting of randomly selected fifty two globular embryoids as well as all the regenerated seventy four plants from Bonnaza navel + rough lemon revealed that twenty six embryoids were tetraploids, and the rest were diploids while 100% regenerated plants were tetraploids. The results inferred that somatic hybrids were more competitive than parental genotypes in the process of plant regeneration. All the regenerated 14 plants from Bonnaza navel + Goutou orange were tetraploids as revealed by chromosome counting. POX isozyme and RAPD analysis verified that the plants from Bonnaza navel + rough lemon were hybrids, and RAPD analysis confirmed the hybridity of those from Bonnaza navel + Goutou orange.