ROLES OF INTEGRIN β1 ON ADHESION BETWEEN DIFFERENT CYCLIC HEPATOCELLULAR CARCINOMA CELLS AND HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
integrin β1在不同细胞周期肝癌细胞与内皮细胞粘附中的作用

To investigate expression of integrin yclic hepatocellular carcinoma cells (HCC) and human umbilical vein endothelial cells (HUVECs), the synchronous G1 and S phase HCC were achieved through thymine-2-desoryriboside and colchicines sequential blockage method and double thymine-2-desoryriboside blockage method, respectively. Expression of integrin ve course were studied by micropipette aspiration technique. The results showed that percentage of each phases of the control HCC cell cycle are 53.51% of G0/G1 phase?11.01% of G2/M phase and 35.48% of S phase, while the synchronous rates of G1 and S phase SMMC-7721 cells amount to 74.09% and 98.29%, respectively. The expressive fluorescent intensity of integrin to HUVEC was significantly lower than the value of S phase cell(P<0.01), but it has no remarkable difference when compared the value of S phase HCC with controlled HCC; the contribution of integrin C. It suggested that HCC would be synchronized preferably in G1 and S phase with thymine-2-desoryriboside and colchicines, the expression of adhesive molecule integrin in HCC's adhesion to HUVEC, and integrin