The clustering effect of purified Epidermal Growth Factor Receptor induced by 50 Hz magnetic field
50 Hz弱磁场诱导离体表皮生长因子受体聚集

By using atomic force microscope (AFM) and transmission electron microscope (TEM), the effects of 50 Hz magnetic field on the clustering of purified A431 EGFR were investigated. Results from AFM and TEM indicated that 30-min field exposure of 50ug/mL EGFR molecules at room temperature, resulted in obvious clusters for a basal size of the molecule particles: the height of apparent individual basal particles increased from (1.42±0.18) nm to (3.08±0.17) nm, and the mean of the half-width (the mean of the diameter) from (21.7±2.2) nm (12.5 nm) to (33.0±4.0) nm (23.0 nm), suggesting a nearly doubled size of EGFR particles. The magnetic field-induced clustering of EGFR was time-dependent. Further, the expression of EGFR on CHL cells was observed and it showed that EGFR was likely up-regulated by MF but no significant difference statistically.