Application of Microsatellite Markers for the Seed Purity Examination of a Hybrid Rice, Gangyou-22
利用微卫星标记鉴定杂交水稻冈优22种子纯度的研究

For seed commercialization of hybrid rice it is necessary to examine the purity of its seeds before field production,because the seed purity is closely related to their heterosis performance and yield increase.In this research,160 microsatellite markers were used for PCR amplification of rice seedling DNAs of Gangyou-22,which is a major hybrid rice in China,and its parents,Gang46A(CMS line)and CDR22(restorer line).A microsatellite marker,RM168,was screened out for its ability to produce polymorphic bands specific to each of the two parents but different from other 22 restorer lines and 9 cultivars.This provides an accurate and efficient method to examine the purity of a hybrid rice at an earlier time.Amplification of DNAs extracted from seeds and application of two microsatellite markers in one PCR system can further simplify the procedure and improve the accuracy of the seed purity examination.